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Polydopamine, a material first isolated from shellfish, could help increase the sensitivity of the tests by as many as 100 to 1 LeSean McCoy Limited Jersey ,000 times.
""Common bioassays are the real workhorses of laboratory experiments and medical tests,"" said Xiaohu Gao, a UW professor of bioengineering. ""By boosting the sensitivity of these tests, we can enable more accurate medical diagnoses earlier in a disease or condition, and enable more certainty and less waste in the research process.""
More sensitive tests would allow scientists to identify pathogens Tremaine Edmunds Limited Jersey , diseases and specific cellular proteins even when these ""biomarkers"" are present at levels far below the detection threshold of today's standard tests. Initial results show polydopamine boosted the accuracy and resolution of these tests for biomarkers of HIV, Zika virus and proteins on cancerous tumors.
Gao led the team that developed this modification for common medical and laboratory assays and recently published their approach, known as enzyme-accelerated signal enhancement, or EASE, in Nature Biomedical Engineering.
EASE centers on the addition of two biochemical components Josh Allen Limited Jersey , dopamine and horseradish peroxidase, or HRP, at a key step. HRP is a common protein enzyme used to speed up the rate of reactions in biomedical research. Gao's team discovered that HRP can connect dopamine molecules together to form the polymer chain polydopamine. Polydopamine, in turn, accumulates on the surfaces of reaction vessels. Once the polydopamine is present John Miller Elite Jersey , researchers can continue the traditional steps of their protocols, but now with a substantially increased test sensitivity.
""Scientists have been trying to improve the accuracy of these common tests for decades, but solutions often involve entirely new protocols or costly pieces of equipment,"" explained Gao. ""Understandably, researchers can be reluctant to invest in unfamiliar protocols or expensive new equipment Shaq Lawson Elite Jersey , but EASE is a simple addition to tried-and-true assays. It's like a software upgrade, instead of changing your operating system.""
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